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Practical validation of sorted fractions was done by xenotransplantation, the result of which was coupled with miRNA expression profiling to deliver stem cell-connected miRNA expression profiles.
To find out whether miRNA are differentially expressed in LSC and HSC, we fractionated 16 AML affected person samples and a few lineage-depleted (Lin–) cord blood (CB) samples working with CD34 and CD38 into 4 populations and subjected Each individual to worldwide miRNA profiling; the stem mobile content material of every portion was functionally assayed by xenotransplantation (Figures 1A and S1A).
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(J) Proliferation calculated by BrdU incorporation assay of CTRL or 126KD transduced 8227 cells in vitro.
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The LSC frequency was diminished in two of three samples upon 126KD (Figures 5C and S5D). Together, these conclusions counsel that 126KD provides heterogeneous responses with LSC functionality and frequency reduced in a subset of AML people.
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Dunia persilatan ibarat lorong goa yang panjang. Tempat ancaman kematian selalu datang dari balik kegelapan. Pertarungan demi pertarungan yang selalu berakhir dengan kematian. Itulah jalan para pendekar membunuh atau di bunuh. Bagiku seorang pendekar mengusai ilmu beladiri bukan mencari kesempurnaan melainkan untuk membela mereka yang lemah dan tak berdaya.
(E) Per cent BrdU incorporation into bulk cultures displaying proliferation of CTRL and 126OE transduced 8227 cells after a while.
Apakah arti kesempurnaan ilmu, jika tak di abadikan untuk kemanusiaan. Namun dalam setiap pertarungan yang berakhir dalam kematian lawan, hanya kehilangan yang kurasakan. Semakin lama semakin dalam. Ada yang hilang dan ada yang ku temukan.
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